In-frame amber stop codon replacement mutagenesis for the directed evolution of proteins containing non-canonical amino acids: identification of residues open to bio-orthogonal modification.
Expanded genetic code approaches are a powerful means to add new and useful chemistry to proteins at defined residues positions.One such use is the introduction of non-biological reactive chemical handles for site-specific biocompatible orthogonal conjugation of proteins.Due to our currently limited information on the impact of non-canonical amino