IN-FRAME AMBER STOP CODON REPLACEMENT MUTAGENESIS FOR THE DIRECTED EVOLUTION OF PROTEINS CONTAINING NON-CANONICAL AMINO ACIDS: IDENTIFICATION OF RESIDUES OPEN TO BIO-ORTHOGONAL MODIFICATION.

In-frame amber stop codon replacement mutagenesis for the directed evolution of proteins containing non-canonical amino acids: identification of residues open to bio-orthogonal modification.

Expanded genetic code approaches are a powerful means to add new and useful chemistry to proteins at defined residues positions.One such use is the introduction of non-biological reactive chemical handles for site-specific biocompatible orthogonal conjugation of proteins.Due to our currently limited information on the impact of non-canonical amino

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Tempo Effects in Different Calculation Types of Period Death Rates

The question as to whether or not tempo effects distort the measurement of period mortality is controversial in recent demographic research.Only few publications, however, illustrate the underlying phenomenon of tempo effects, namely that the period death rate may increase although the mortality of all cohorts living during the analyzed period has

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The Significance of Work Motivation: The Impact of Organizational Culture and Work Environment on Employee Performance

The objective of this study is to ascertain and examine the impact of Organizational Culture and Work Environment on Employee Performance, with Work Motivation serving as Cap an intermediary factor.This study employs quantitative methodologies.The study sample included all individuals employed by the hospital.The study used a purposive sampling str

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